Solana, J. C., Moreno, J., Iborra, S., Soto, M., & Requena, J. M. (2022). Live attenuated vaccines, a favorable strategy to provide long-term immunity against protozoan diseases. Trends in Parasitology, 38(4), 316–334. https://doi.org/10.1016/j.pt.2021.11.004

Márvez, E. R., Segura, C. A. R., Requena, J. M., & Puerta, C. J. (2022). A dataset of proteins associated with Trypanosoma cruzi LYT1 mRNAs. Data in Brief, 41, 107953. https://doi.org/10.1016/j.dib.2022.107953

Solana, J. C., Bernardo, L., Moreno, J., Aguado, B., & Requena, J. M. (2022). The Astonishing Large Family of HSP40/DnaJ Proteins Existing in Leishmania. Genes, 13(5), 742. https://doi.org/10.3390/genes13050742

Solana, J. C., Chicharro, C., García, E., Aguado, B., Moreno, J., & Requena, J. M. (2022). Assembly of a Large Collection of Maxicircle Sequences and Their Usefulness for Leishmania Taxonomy and Strain Typing. Genes, 13(6), 1070. https://doi.org/10.3390/genes13061070

Soto, M., Ramírez, L., Solana, J. C., Cook, E. C. L., Hernández-García, E., Requena, J. M., & Iborra, S. (2021). Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice. Microorganisms, 9(2), 363. https://doi.org/10.3390/microorganisms9020363

Fernández, L., Solana, J. C., Sánchez, C., Jiménez, M. Á., Requena, J. M., Coler, R., Reed, S. G., Valenzuela, J. G., Kamhawi, S., Oliveira, F., Fichera, E., Glueck, R., Bottazzi, M. E., Gupta, G., Cecilio, P., Pérez-Cabezas, B., Cordeiro-da-Silva, A., Gradoni, L., Carrillo, E., & Moreno, J. (2021). Protective Efficacy in a Hamster Model of a Multivalent Vaccine for Human Visceral Leishmaniasis (MuLeVaClin) Consisting of the KMP11, LEISH-F3+, and LJL143 Antigens in Virosomes, Plus GLA-SE Adjuvant. Microorganisms, 9(11), 2253. https://doi.org/10.3390/microorganisms9112253

Solana, J. C., Ramírez, L., Cook, E. C., Hernández-García, E., Sacristán, S., Martín, M. E., Manuel González, V., Reguera, R. M., Balaña-Fouce, R., Fresno, M., Requena, J. M., Iborra, S., & Soto, M. (2020). Subcutaneous Immunization of Leishmania HSP70-II Null Mutant Line Reduces the Severity of the Experimental Visceral Leishmaniasis in BALB/c Mice. Vaccines, 8(1), 141. https://doi.org/10.3390/vaccines8010141

Ruiz-Márvez, E., Ramírez, C. A., Rodríguez, E. R., Flórez, M. M., Delgado, G., Guzmán, F., Gómez-Puertas, P., Requena, J. M., & Puerta, C. J. (2020). Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi. International Journal of Molecular Sciences, 21(7), 2432. https://doi.org/10.3390/ijms21072432

Sanchiz, Á., Morato, E., Rastrojo, A., Camacho, E., González-de la Fuente, S., Marina, A., Aguado, B., & Requena, J. M. (2020). The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations. Genes, 11(9), 1036. https://doi.org/10.3390/genes11091036

Rastrojo, A., Corvo, L., Lombraña, R., Solana, J. C., Aguado, B., & Requena, J. M. (2019). Analysis by RNA-seq of transcriptomic changes elicited by heat shock in Leishmania major. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-43354-9

Camacho, E., Rastrojo, A., Sanchiz, Á., González-de la Fuente, S., Aguado, B., & Requena, J. M. (2019). Leishmania Mitochondrial Genomes: Maxicircle Structure and Heterogeneity of Minicircles. Genes, 10(10), 758. https://doi.org/10.3390/genes10100758

Camacho, E., González-de la Fuente, S., Rastrojo, A., Peiró-Pastor, R., Solana, J. C., Tabera, L., Gamarro, F., Carrasco-Ramiro, F., Requena, J. M., & Aguado, B. (2019). Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-42511-4

Ruiz, E., Ramírez, C. A., Casas, J. C., Ospina, M. I., Requena, J. M., & Puerta, C. J. (2018). Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing. Universitas Scientiarum, 23(2), 267–290. https://doi.org/10.11144/javeriana.sc23-2.cotm

Ruiz, E., Ramírez, C. A., Nocua, P., Requena, J. M., & Puerta, C. J. (2018). Identificación de proteínas reguladoras de la expresión génica en tripanosomátidos. Revista de La Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 42(165), 306. https://doi.org/10.18257/raccefyn.671

Solana, J. C., Ramírez, L., Corvo, L., de Oliveira, C. I., Barral-Netto, M., Requena, J. M., Iborra, S., & Soto, M. (2017). Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models. PLOS Neglected Tropical Diseases, 11(5), e0005644. https://doi.org/10.1371/journal.pntd.0005644

Requena, J. M., Rastrojo, A., Garde, E., López, M. C., Thomas, M. C., & Aguado, B. (2017). Genomic cartography and proposal of nomenclature for the repeated, interspersed elements of the Leishmania major SIDER2 family and identification of SIDER2-containing transcripts. Molecular and Biochemical Parasitology, 212, 9–15. https://doi.org/10.1016/j.molbiopara.2016.12.009

Requena, J. M., Rastrojo, A., Garde, E., López, M. C., Thomas, M. C., & Aguado, B. (2017). Dataset for distribution of SIDER2 elements in the Leishmania major genome and transcriptome. Data in Brief, 11, 39–43. https://doi.org/10.1016/j.dib.2017.01.001

González-de la Fuente, S., Peiró-Pastor, R., Rastrojo, A., Moreno, J., Carrasco-Ramiro, F., Requena, J. M., & Aguado, B. (2017). Resequencing of the Leishmania infantum (strain JPCM5) genome and de novo assembly into 36 contigs. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-18374-y

Alonso, G., Rastrojo, A., López-Pérez, S., Requena, J. M., & Aguado, B. (2016). Resequencing and assembly of seven complex loci to improve the Leishmania major (Friedlin strain) reference genome. Parasites & Vectors, 9(1). https://doi.org/10.1186/s13071-016-1329-4

Fernández-Orgiler, A., Martínez-Jiménez, M. I., Alonso, A., Alcolea, P. J., Requena, J. M., Thomas, M. C., Blanco, L., & Larraga, V. (2016). A putativeLeishmaniaDNA polymerase theta protects the parasite against oxidative damage. Nucleic Acids Research, 44(10), 4855–4870. https://doi.org/10.1093/nar/gkw346

Requena, J. M., Montalvo, A. M., & Fraga, J. (2015). Molecular Chaperones ofLeishmania: Central Players in Many Stress-Related and -Unrelated Physiological Processes. BioMed Research International, 2015, 1–21. https://doi.org/10.1155/2015/301326

Nocua, P. A., Ramirez, C. A., Barreto, G. E., González, J., Requena, J. M., & Puerta, C. J. (2014). Leishmania braziliensis replication protein A subunit 1: molecular modelling, protein expression and analysis of its affinity for both DNA and RNA. Parasites & Vectors, 7(1). https://doi.org/10.1186/s13071-014-0573-8

Ramírez, C. A., Dea-Ayuela, M. A., Gutiérrez-Blázquez, M. D., Bolas-Fernández, F., Requena, J. M., & Puerta, C. J. (2013). Identification of proteins interacting with HSP70 mRNAs in Leishmania braziliensis. Journal of Proteomics, 94, 124–137. https://doi.org/10.1016/j.jprot.2013.09.008

Prada, C. F., Álvarez-Velilla, R., Balaña-Fouce, R., Prieto, C., Calvo-Álvarez, E., Escudero-Martínez, J. M., Requena, J. M., Ordóñez, C., Desideri, A., Pérez-Pertejo, Y., & Reguera, R. M. (2013). Gimatecan and other camptothecin derivatives poison Leishmania DNA-topoisomerase IB leading to a strong leishmanicidal effect. Biochemical Pharmacology, 85(10), 1433–1440. https://doi.org/10.1016/j.bcp.2013.02.024

Rastrojo, A., Carrasco-Ramiro, F., Martín, D., Crespillo, A., Reguera, R. M., Aguado, B., & Requena, J. M. (2013). The transcriptome of Leishmania majorin the axenic promastigote stage: transcript annotation and relative expression levels by RNA-seq. BMC Genomics, 14(1). https://doi.org/10.1186/1471-2164-14-223

Ramírez, C. A., Requena, J. M., & Puerta, C. J. (2013). Alpha tubulin genes from Leishmania braziliensis:genomic organization, gene structure and insights on their expression. BMC Genomics, 14(1). https://doi.org/10.1186/1471-2164-14-454

Fraga, J., Montalvo, A. M., Van der Auwera, G., Maes, I., Dujardin, J.-C., & Requena, J. M. (2013). Evolution and species discrimination according to the Leishmania heat-shock protein 20 gene. Infection, Genetics and Evolution, 18, 229–237. https://doi.org/10.1016/j.meegid.2013.05.020

Balaña-Fouce, R., Prada, C. F., Requena, J. M., Cushman, M., Pommier, Y., Álvarez-Velilla, R., Escudero-Martínez, J. M., Calvo-Álvarez, E., Pérez-Pertejo, Y., & Reguera, R. M. (2012). Indotecan (LMP400) and AM13-55: Two Novel Indenoisoquinolines Show Potential for Treating Visceral Leishmaniasis. Antimicrobial Agents and Chemotherapy, 56(10), 5264–5270. https://doi.org/10.1128/aac.00499-12

Calvo-Álvarez, E., Guerrero, N. A., Álvarez-Velilla, R., Prada, C. F., Requena, J. M., Punzón, C., Llamas, M. Á., Arévalo, F. J., Rivas, L., Fresno, M., Pérez-Pertejo, Y., Balaña-Fouce, R., & Reguera, R. M. (2012). Appraisal of a Leishmania major Strain Stably Expressing mCherry Fluorescent Protein for Both In Vitro and In Vivo Studies of Potential Drugs and Vaccine against Cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, 6(11), e1927. https://doi.org/10.1371/journal.pntd.0001927

Requena, J. M., Chicharro, C., García, L., Parrado, R., Puerta, C. J., & Cañavate, C. (2012). Sequence analysis of the 3’-untranslated region of HSP70 (type I) genes in the genus Leishmania: its usefulness as a molecular marker for species identification. Parasites & Vectors, 5(1). https://doi.org/10.1186/1756-3305-5-87

Ali, N., Mekuria, A. H., Requena, J. M., & Engwerda, C. (2012). Immunity to Visceral Leishmaniasis. Journal of Tropical Medicine, 2012, 1–2. https://doi.org/10.1155/2012/780809

Requena, J., M. (2011). Lights and shadows on gene organization and regulation of gene expression in Leishmania. Frontiers in Bioscience, 16(1), 2069. https://doi.org/10.2741/3840

Carrión, J., Folgueira, C., Soto, M., Fresno, M., & Requena, J. M. (2011). Leishmania infantum HSP70-II null mutant as candidate vaccine against leishmaniasis: a preliminary evaluation. Parasites & Vectors, 4(1). https://doi.org/10.1186/1756-3305-4-150

Ramírez, C. A., Requena, J. M., & Puerta, C. J. (2011). Identification of the HSP70-II gene in Leishmania braziliensis HSP70 locus: genomic organization and UTRs characterization. Parasites & Vectors, 4(1). https://doi.org/10.1186/1756-3305-4-166

Folgueira, C., Martinez-Bonet, M., & Requena, J. M. (2010). The Leishmania infantum PUF proteins are targets of the humoral response during visceral leishmaniasis. BMC Research Notes, 3(1), 13. https://doi.org/10.1186/1756-0500-3-13

Ramírez, C., Puerta, C., & Requena, J. M. (2010). Evidence of RNA editing in Leishmania braziliensis promastigotes. Parasitology Research, 108(3), 731–739. https://doi.org/10.1007/s00436-010-2190-6

Carrillo, E., Crusat, M., Nieto, J., Chicharro, C., Thomas, M. del C., Martínez, E., Valladares, B., Cañavate, C., Requena, J. M., López, M. C., Alvar, J., & Moreno, J. (2008). Immunogenicity of HSP-70, KMP-11 and PFR-2 leishmanial antigens in the experimental model of canine visceral leishmaniasis. Vaccine, 26(15), 1902–1911. https://doi.org/10.1016/j.vaccine.2008.01.042

Folgueira, C., & Carrión, J. (2008). Effects of the disruption of the HSP70-II gene on the growth, morphology, and virulence of Leishmania infantum promastigotes [JB]. International Microbiology, 11, 81–89. https://doi.org/10.2436/20.1501.01.47

Requena, J. M., Folgueira, C., López, M. C., & Thomas, M. C. (2008). The SIDER2 elements, interspersed repeated sequences that populate the Leishmania genomes, constitute subfamilies showing chromosomal proximity relationship. BMC Genomics, 9(1), 263. https://doi.org/10.1186/1471-2164-9-263

Folgueira, C., & Requena, J. M. (2007). A postgenomic view of the heat shock proteins in kinetoplastids. FEMS Microbiology Reviews, 31(4), 359–377. https://doi.org/10.1111/j.1574-6976.2007.00069.x

Folgueira, C., & Requena, J. M. (2007). Pitfalls of the CAT reporter gene for analyzing translational regulation in Leishmania. Parasitology Research, 101(5), 1449–1452. https://doi.org/10.1007/s00436-007-0640-6

CARRIÓN, J., NIETO, A., IBORRA, S., INIESTA, V., SOTO, M., FOLGUEIRA, C., ABANADES, D. R., REQUENA, J. M., & ALONSO, C. (2006). Immunohistological features of visceral leishmaniasis in BALB/c mice. Parasite Immunology, 28(5), 173–183. Portico. https://doi.org/10.1111/j.1365-3024.2006.00817.x

FOLGUEIRA, C., CAÑAVATE, C., CHICHARRO, C., & REQUENA, J. M. (2006). Genomic organization and expression of the HSP70 locus in New and Old World Leishmania species. Parasitology, 134(3), 369–377. https://doi.org/10.1017/s0031182006001570

Quijada, L., Soto, M., & Requena, J. M. (2005). Genomic DNA macroarrays as a tool for analysis of gene expression in Leishmania. Experimental Parasitology, 111(1), 64–70. https://doi.org/10.1016/j.exppara.2005.04.006

Folgueira, C., Quijada, L., Soto, M., Abanades, D. R., Alonso, C., & Requena, J. M. (2005). The Translational Efficiencies of the Two Leishmania infantum HSP70 mRNAs, Differing in Their 3′-Untranslated Regions, Are Affected by Shifts in the Temperature of Growth through Different Mechanisms. Journal of Biological Chemistry, 280(42), 35172–35183. https://doi.org/10.1074/jbc.m505559200

Parody, N., Soto, M., Requena, J. M., & Alonso, C. (2004). Adjuvant guided polarization of the immune humoral response against a protective multicomponent antigenic protein (Q) from Leishmania infantum. A CpG + Q mix protects Balb/c mice from infection. Parasite Immunology, 26(6–7), 283–293. Portico. https://doi.org/10.1111/j.0141-9838.2004.00711.x

SOTO, M., IBORRA, S., QUIJADA, L., FOLGUEIRA, C., ALONSO, C., & REQUENA, J. M. (2004). Cell-cycle-dependent translation of histone mRNAs is the key control point for regulation of histone biosynthesis in Leishmania infantum. Biochemical Journal, 379(3), 617–625. https://doi.org/10.1042/bj20031522

Larreta, R., Soto, M., Quijada, L., Folgueira, C., Abanades, D. R., Alonso, C., & Requena, J. M. (2004). The expression of HSP83 genes in Leishmania infantum is affected by temperature and by stage-differentiation and is regulated at the levels of mRNA stability and translation. BMC Molecular Biology, 5(1). https://doi.org/10.1186/1471-2199-5-3

IBORRA, S. (2004). Vaccination with a plasmid DNA cocktail encoding the nucleosomal histones of Leishmania confers protection against murine cutaneous leishmaniosis. Vaccine, 22(29–30), 3865–3876. https://doi.org/10.1016/j.vaccine.2004.04.015

Requena, J. M., Iborra, S., Carrión, J., Alonso, C., & Soto, M. (2004). Recent advances in vaccines for leishmaniasis. Expert Opinion on Biological Therapy, 4(9), 1505–1517. https://doi.org/10.1517/14712598.4.9.1505

Villa, H., Pérez‐Pertejo, Y., García‐Estrada, C., Reguera, R. M., Requena, J. M., Tekwani, B. L., Balaña‐Fouce, R., & Ordóñez, D. (2003). Molecular and functional characterization of adenylate kinase 2 gene fromLeishmania donovani. European Journal of Biochemistry, 270(21), 4339–4347. Portico. https://doi.org/10.1046/j.1432-1033.2003.03826.x

SOTO, M., QUIJADA, L., LARRETA, R., IBORRA, S., ALONSO, C., & REQUENA, J. M. (2003). Leishmania infantum possesses a complex family of histone H2A genes: structural characterization and analysis of expression. Parasitology, 127(2), 95–105. https://doi.org/10.1017/s0031182003003445

García-Estrada, C. (2003). Identification of a gene in Leishmania infantum encoding a protein that contains a SP-RING/MIZ zinc finger domain. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1629(1–3), 44–52. https://doi.org/10.1016/j.bbaexp.2003.07.001

Molano, I., Alonso, M. G., Mirón, C., Redondo, E., Requena, J. M., Soto, M., Nieto, C. G., & Alonso, C. (2003). A Leishmania infantum multi-component antigenic protein mixed with live BCG confers protection to dogs experimentally infected with L. infantum. Veterinary Immunology and Immunopathology, 92(1–2), 1–13. https://doi.org/10.1016/s0165-2427(02)00315-x

Requena, J. M., Quijada, L., Soto, M., & Alonso, C. (2003). Conserved nucleotides surrounding the trans-splicing acceptor site and the translation initiation codon in Leishmania genes. Experimental Parasitology, 103(1–2), 78–81. https://doi.org/10.1016/s0014-4894(03)00061-4

Quijada, L., Soto, M., Alonso, C., & Requena, J. M. (2003). High-efficiency plating method for Leishmania infantum. Molecular and Biochemical Parasitology, 130(2), 139–141. https://doi.org/10.1016/s0166-6851(03)00159-2

Larreta, R. (2002). Antigenic properties of the Leishmania infantum GRP94 and mapping of linear B-cell epitopes. Immunology Letters, 80(3), 199–205. https://doi.org/10.1016/s0165-2478(01)00331-5

Echeverria, P., Dran, G., Pereda, G., Rico, A. I., Requena, J. M., Alonso, C., Guarnera, E., & Angel, S. O. (2001). Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination. Immunology Letters, 76(2), 107–110. https://doi.org/10.1016/s0165-2478(01)00179-1

Soto, M., Alonso, C., & Requena, J. M. (2000). TheLeishmania infantumacidic ribosomal protein LiP2a induces a prominent humoral responsein vivoand stimulates cell proliferationin vitroand interferon-gamma (IFN-γ) production by murine splenocytes. Clinical and Experimental Immunology, 122(2), 212–218. https://doi.org/10.1046/j.1365-2249.2000.01372.x

Larreta, R., Soto, M., Alonso, C., & Requena, J. M. (2000). Leishmania infantum: Gene Cloning of the GRP94 Homologue, Its Expression as Recombinant Protein, and Analysis of Antigenicity. Experimental Parasitology, 96(2), 108–115. https://doi.org/10.1006/expr.2000.4553

Requena, J. M., Soto, M., Doria, M. D., & Alonso, C. (2000). Immune and clinical parameters associated with Leishmania infantum infection in the golden hamster model. Veterinary Immunology and Immunopathology, 76(3–4), 269–281. https://doi.org/10.1016/s0165-2427(00)00221-x

Quijada, L., Soto, M., Alonso, C., & Requena, J. M. (2000). Identification of a putative regulatory element in the 3′-untranslated region that controls expression of HSP70 in Leishmania infantum. Molecular and Biochemical Parasitology, 110(1), 79–91. https://doi.org/10.1016/s0166-6851(00)00258-9

SOTO, M., QUIJADA, L., ALONSO, C., & REQUENA, J. M. (2000). Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control. Biochemical Journal, 346(1), 99–105. https://doi.org/10.1042/bj3460099

Requena, J. M., Alonso, C., & Soto, M. (2000). Evolutionarily Conserved Proteins as Prominent Immunogens during Leishmania Infections. Parasitology Today, 16(6), 246–250. https://doi.org/10.1016/s0169-4758(00)01651-3

Planelles, L., Marañón, C., Requena, J. M., & López, M. C. (1999). Phage Recovery by Electroporation of Naked DNA into Host Cells Avoids the Use of Packaging Extracts. Analytical Biochemistry, 267(1), 234–235. https://doi.org/10.1006/abio.1998.2963

Rico, A. I., Angel, S. O., Alonso, C., & Requena, J. M. (1999). Immunostimulatory properties of the Leishmania infantum heat shock proteins HSP70 and HSP83. Molecular Immunology, 36(17), 1131–1139. https://doi.org/10.1016/s0161-5890(99)00136-4

SOTO, M., REQUENA, J. M., QUIJADA, L., PEREZ, M. J., NIETO, C. G., GUZMAN, F., PATARROYO, M. E., & ALONSO, C. (1999). Antigenicity of theLeishmania infantumhistones H2B and H4 during canine viscerocutaneous leishmaniasis. Clinical and Experimental Immunology, 115(2), 342–349. https://doi.org/10.1046/j.1365-2249.1999.00796.x

Nieto, C. G., Garcı́a-Alonso, M., Requena, J. M., Mirón, C., Soto, M., Alonso, C., & Navarrete, I. (1999). Analysis of the humoral immune response against total and recombinant antigens of Leishmania infantum: correlation with disease progression in canine experimental leishmaniasis. Veterinary Immunology and Immunopathology, 67(2), 117–130. https://doi.org/10.1016/s0165-2427(98)00213-x

Carriazo, C. S., Sembaj, A., Aguerri, A. M., Requena, J. M., Alonso, C., Búa, J., Ruiz, A., Segura, E., & Barral, J. M. (1998). Polymerase Chain Reaction Procedure to Detect Trypanosoma cruzi in Blood Samples from Chronic Chagasic Patients. Diagnostic Microbiology and Infectious Disease, 30(3), 183–186. https://doi.org/10.1016/s0732-8893(97)00238-1

Jiménez‐Ruiz, A., Boceta, C., Bonay, P., Requena, J. M., & Alonso, C. (1998). Cloning, sequencing, and expression of the PSA genes from Leishmania infantum. European Journal of Biochemistry, 251(1–2), 389–397. Portico. https://doi.org/10.1046/j.1432-1327.1998.2510389.x

Quijada, L. (1998). Analysis of the antigenic properties of the L. infantum Hsp70: design of synthetic peptides for specific serodiagnosis of human leishmaniasis. Immunology Letters, 63(3), 169–174. https://doi.org/10.1016/s0165-2478(98)00071-6

Soto, M., Quijada, L., Alonso, C., & Requena, J. M. (1997). Molecular cloning and analysis of expression of the Leishmania infantum histone H4 genes1Note: Nucleotide sequence data reported in this paper have been submitted to the EMBL/GenBank/DDBJ databases with the accession numbers Y13915 (cDNA LiH4-1) and Y13916 (cDNA LiH4-2).1. Molecular and Biochemical Parasitology, 90(2), 439–447. https://doi.org/10.1016/s0166-6851(97)00178-3

Berberich, C., Requena, J. M., & Alonso, C. (1997). Cloning of Genes and Expression and Antigenicity Analysis of theLeishmania infantumKMP-11 Protein. Experimental Parasitology, 85(1), 105–108. https://doi.org/10.1006/expr.1996.4120

ARAYA, J., CANO, M. I., GOMES, H. B. M., NOVAK, E. M., REQUENA, J. M., ALONSO, C., LEVIN, M. J., GUEVARA, P., RAMIREZ, J. L., & DA SILVEIRA, J. F. (1997). Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi. Parasitology, 115(6), 563–570. https://doi.org/10.1017/s0031182097001674

Quijada, L., Soto, M., Alonso, C., & Requena, J. M. (1997). Analysis of Post-transcriptional Regulation Operating on Transcription Products of the Tandemly Linked Leishmania infantum hsp70 Genes. Journal of Biological Chemistry, 272(7), 4493–4499. https://doi.org/10.1074/jbc.272.7.4493

Requena, J. M., Soto, M., Quijada, L., Carrillo, G., & Alonso, C. (1997). A region containing repeated elements is associated with transcriptional termination of Leishmania infantum ribosomal RNA genes1Note: Nucleotide sequences data reported in this paper are available in the EMBL, GenBank™ and DDJB data bases under the accession number X99114.1. Molecular and Biochemical Parasitology, 84(1), 101–110. https://doi.org/10.1016/s0166-6851(96)02785-5

Requena, J. M., López, M. C., & Alonso, C. (1996). Genomic repetitive DNA elements of Trypanosoma cruzi. Parasitology Today, 12(7), 279–283. https://doi.org/10.1016/0169-4758(96)10024-7

Angel, S. O., Requena, J. M., Soto, M., Criago, D., & Alonso, C. (1996). During canine leishmaniasis a protein belonging to the 83-kDa heat-shock protein family elicits a strong humoral response. Acta Tropica, 62(1), 45–56. https://doi.org/10.1016/s0001-706x(96)00020-4

Cano, M. I., Gruber, A., Vazquez, M., Cortés, A., Levin, M. J., González, A., Degrave, W., Rondinelli, E., Zingales, B., Ramirez, J., Alonso, C., Requena, JoséM., & Silveira, J. da. (1995). Molecular karyotype of clone CL Brener chosen for the Trypanosoma cruzi Genome Project. Molecular and Biochemical Parasitology, 71(2), 273–278. https://doi.org/10.1016/0166-6851(95)00066-a

Soto, M., Requena, J. M., Quijada, L., García, M., Guzman, F., Patarroyo, M. E., & Alonso, C. (1995). Mapping of the linear antigenic determinants from the Leishmania infantum histone H2A recognized by sera from dogs with leishmaniasis. Immunology Letters, 48(3), 209–214. https://doi.org/10.1016/0165-2478(95)02473-5

Soto, M., Requena, J. M., Quijada, L., Guzman, F., Patarroyo, M. E., & Alonso, C. (1995). Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis. Immunology Letters, 48(1), 23–28. https://doi.org/10.1016/0165-2478(95)02436-0

Soto, M., Requena, J. M., Moreira, D., & Alonso, C. (1995). Assignment of genes toLeishmania infantumchromosomes: karyotype and ploidy. FEMS Microbiology Letters, 129(1), 27–32. https://doi.org/10.1111/j.1574-6968.1995.tb07552.x

Soto, M., Requena, JoséM., Morales, G., & Alonso, C. (1994). The Leishmania infantum histone H3 possesses an extremely divergent N-terminal domain. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1219(2), 533–535. https://doi.org/10.1016/0167-4781(94)90082-5

Requena, J. M., Martin, F., Soto, M., López, M. C., & Alonso, C. (1994). Characterization of a short interspersed reiterated DNA sequence of Trypanosoma cruzi located at the 3′-end of a poly(A)+ transcript. Gene, 146(2), 245–250. https://doi.org/10.1016/0378-1119(94)90300-x

Requena, J. M., Soto, M., Guzman, F., Maekelt, A., Noya, O., Patarroyo, M. E., & Alonso, C. (1993). Mapping of antigenic determinants of the T. cruzi HSP70 in chagasic and healthy individuals. Molecular Immunology, 30(12), 1115–1121. https://doi.org/10.1016/0161-5890(93)90158-8

Soto, M., Requena, J. M., & Alonso, C. (1993). Isolation, characterization and analysis of the expression of the Leishmania ribosomal PO protein genes. Molecular and Biochemical Parasitology, 61(2), 265–274. https://doi.org/10.1016/0166-6851(93)90072-6

Martin, F., Requena, J. M., Martin, J., Alonso, C., & Lopez, M. C. (1993). Cytoplasmic-Nuclear Translocation of the HSP70 Protein during Environmental Stress in Trypanosoma cruzi. Biochemical and Biophysical Research Communications, 196(3), 1155–1162. https://doi.org/10.1006/bbrc.1993.2372

Pérez, J. M., Requena, J. M., Craciunescu, D., Doadrio, J. C., & Alonso, C. (1993). Binding of Pt-pentamidine to nucleosomal DNA. Studies of the antiproliferative activity of the drug against human cancer cells. Chemico-Biological Interactions, 89(1), 61–72. https://doi.org/10.1016/0009-2797(93)03199-5

Requena, J. M., Soto, M., & Alonso, C. (1993). Bacteriophage λ: long-term stored stocks ready for lysis. Trends in Genetics, 9(1), 4. https://doi.org/10.1016/0168-9525(93)90052-j

Requena, JoséM., Jimenez-Ruiz, A., Soto, M., Assiego, R., Santarén, J. F., Lopez, M. C., Patarroyo, M. E., & Alonso, C. (1992). Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase. Molecular and Biochemical Parasitology, 53(1–2), 201–211. https://doi.org/10.1016/0166-6851(92)90022-c

SOTO, M., REQUENA, J. M., GOMEZ, L. C., NAVARRETE, I., & ALONSO, C. (1992). Molecular characterization of a Leishmania donovani infantum antigen identified as histone H2A. European Journal of Biochemistry, 205(1), 211–216. Portico. https://doi.org/10.1111/j.1432-1033.1992.tb16770.x

Requena, J. M., Jimenez-Ruiz, A., Soto, M., Lopez, M. C., & Alonso, C. (1992). Characterization of a highly repeated interspersed DNA sequence of Trypanosoma cruzi: its potential use in diagnosis and strain classification. Molecular and Biochemical Parasitology, 51(2), 271–280. https://doi.org/10.1016/0166-6851(92)90077-w

Soto, M., Requena, J. M., Jimenez-Ruiz, A., & Alonso, C. (1991). The mRNA coding for the nucleosomal protein H2A ofLeishmaniais polyadenylated and has stem-loops at the 3′ end. Nucleic Acids Research, 19(16), 4554–4554. https://doi.org/10.1093/nar/19.16.4554

Manuel Pérez, J., Del Carmen Navarro-Ranninger, M., María Requena, J., Jimenéz-Ruiz, A., Parrondo, E., Craciunescu, D., Carlos López, M., & Alonso, C. (1991). DNA binding properties and antileukemic (L1210) activity of a Pt-pentamidine complex. Chemico-Biological Interactions, 77(3), 341–355. https://doi.org/10.1016/0009-2797(91)90042-6

Jimenez-Ruiz, A., Requena, J. M., Lopez, M. C., & Alonso, C. (1991). A potential Z-DNA-forming sequence is located between two transcription units alternatively expressed during development of Drosophila hydei. Proceedings of the National Academy of Sciences, 88(1), 31–35. https://doi.org/10.1073/pnas.88.1.31

Laran, E., Requena, J. M., Jimenez-Ruiz, A., Lopez, M. C., & Alonso, C. (1990). The heat shock protein hsp70 binds in vivo to subregions 2-48BC and 3-58D of the polytene chromosomes ofDrosophila hydei. Chromosoma, 99(5), 315–320. https://doi.org/10.1007/bf01731718

Morales, G., Requena, J., Jimenez-Ruiz, A., Lopez, M. C., Ugarte, M., & Alonso, C. (1990). Sequence and expression of the Drosophila phenylalanine hydroxylase mRNA. Gene, 93(2), 213–219. https://doi.org/10.1016/0378-1119(90)90227-i

Jimenez–Ruiz, A., Requena, J. M., Lancillotti, F., Morales, G., Lopez, M. C., & Alonso, C. (1989). Molecular cloning of a Drosophila potential Z-DNA forming sequence hybridizingin situto a developmentally regulated subdivision of the polytene chromosomes. Nucleic Acids Research, 17(12), 4579–4588. https://doi.org/10.1093/nar/17.12.4579

Requena, J. M., Lopez, M. C., Jimenez-Ruiz, A., Morales, G., & Alonso, C. (1989). Complete nucleotide sequence of the hsp70 gene ofT.cruzi. Nucleic Acids Research, 17(2), 797–797. https://doi.org/10.1093/nar/17.2.797

Requena, J. M., López, M. C., Jimnez-Ruiz, A., de la Torre, J. C., & Alonso, C. (1988). A head-to-tail tandem organization of hsp70 genes inTrypanosoma cruzi. Nucleic Acids Research, 16(4), 1393–1406. https://doi.org/10.1093/nar/16.4.1393