Bioinformatics and Evolutionary Genomics

Institution: Instituto de Biología Molecular y Celular de Plantas Primo Yúfera (IBMCP)

Members (researchers): José María Requena Rolanía, Aureliano Bombarely

Home page: https://bombarelylab.com/
Contact email: abombarely@ibmcp.upv.es

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Research topics:
Publications

Valverde, S., Vidiella, B., Martínez-Redondo, G. I., Duran-Nebreda, S., Fernández, R., Bombarely, A., Rojas, A. M., & Bentley, R. A. (2025). Structural Changes in Gene Ontology Reveal Modular and Complex Representations of Biological Function. Molecular Biology and Evolution, 42(6). https://doi.org/10.1093/molbev/msaf148

Baraja-Fonseca, V., Arrones, A., Vilanova, S., Plazas, M., Prohens, J., Bombarely, A., & Gramazio, P. (2024). Benchmarking of Low Coverage Sequencing Workflows for Precision Genotyping in Eggplant. https://doi.org/10.1101/2024.10.24.619843

Soares, L. S., Bombarely, A., & Freitas, L. B. (2024). How many species are there? Lineage diversification and hidden speciation in Solanaceae from highland grasslands in southern South America. Annals of Botany, 134(7), 1291–1305. https://doi.org/10.1093/aob/mcae144

Talavera, A., Soorni, A., Bombarely, A., Matas, A. J., & Hormaza, J. I. (2023). Author Correction: Genome-Wide SNP discovery and genomic characterization in avocado (Persea americana Mill.). Scientific Reports, 13(1). https://doi.org/10.1038/s41598-023-29346-w

D’Andrea, L., Perez de Souza, L., Fernie, A. R., & Bombarely, A. (2023). MetPlast: an R package to evaluate Metabolic Plasticity using Information Theory statistical framework. https://doi.org/10.1101/2023.04.28.472208

Gomez, S. K., Maurya, A. K., Irvin, L., Kelly, M. P., Schoenherr, A. P., Huguet-Tapia, J. C., & Bombarely, A. (2023). A snapshot of the transcriptome ofMedicago truncatula(Fabales: Fabaceae) shoots and roots in response to an arbuscular mycorrhizal fungus and the pea aphid (Acyrthosiphon pisum) (Hemiptera: Aphididae). Environmental Entomology, 52(4), 667–680. https://doi.org/10.1093/ee/nvad070

Manrique, S., Cavalleri, A., Guazzotti, A., Villarino, G. H., Simonini, S., Bombarely, A., Higashiyama, T., Grossniklaus, U., Mizzotti, C., Pereira, A. M., Coimbra, S., Sankaranarayanan, S., Onelli, E., Masiero, S., Franks, R. G., & Colombo, L. (2023). HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation. Plant Physiology, 194(4), 2117–2135. https://doi.org/10.1093/plphys/kiad629

Yan, H., Haak, D. C., Li, S., Huang, L., & Bombarely, A. (2022). Exploring transposable element-based markers to identify allelic variations underlying agronomic traits in rice. Plant Communications, 3(3), 100270. https://doi.org/10.1016/j.xplc.2021.100270

Solana, J. C., Moreno, J., Iborra, S., Soto, M., & Requena, J. M. (2022). Live attenuated vaccines, a favorable strategy to provide long-term immunity against protozoan diseases. Trends in Parasitology, 38(4), 316–334. https://doi.org/10.1016/j.pt.2021.11.004

Márvez, E. R., Segura, C. A. R., Requena, J. M., & Puerta, C. J. (2022). A dataset of proteins associated with Trypanosoma cruzi LYT1 mRNAs. Data in Brief, 41, 107953. https://doi.org/10.1016/j.dib.2022.107953

Solana, J. C., Bernardo, L., Moreno, J., Aguado, B., & Requena, J. M. (2022). The Astonishing Large Family of HSP40/DnaJ Proteins Existing in Leishmania. Genes, 13(5), 742. https://doi.org/10.3390/genes13050742

Solana, J. C., Chicharro, C., García, E., Aguado, B., Moreno, J., & Requena, J. M. (2022). Assembly of a Large Collection of Maxicircle Sequences and Their Usefulness for Leishmania Taxonomy and Strain Typing. Genes, 13(6), 1070. https://doi.org/10.3390/genes13061070

Cucinotta, M., Cavalleri, A., Guazzotti, A., Astori, C., Manrique, S., Bombarely, A., Oliveto, S., Biffo, S., Weijers, D., Kater, M. M., & Colombo, L. (2021). Alternative Splicing Generates a MONOPTEROS Isoform Required for Ovule Development. Current Biology, 31(4), 892-899.e3. https://doi.org/10.1016/j.cub.2020.11.026

Garzón-Martínez, G. A., García-Arias, F. L., Enciso-Rodríguez, F. E., Soto-Suárez, M., González, C., Bombarely, A., Barrero, L. S., & Osorio Guarín, J. A. (2021). Combining transcriptome analysis and GWAS for identification and validation of marker genes in thePhysalis peruviana-Fusarium oxysporumpathosystem. PeerJ, 9, e11135. Portico. https://doi.org/10.7717/peerj.11135

Bombarely, A., Doulis, A. G., Lambrou, K. K., Zioutis, C., Margaritis, E., & Koubouris, G. (2021). Elucidation of the Origin of the Monumental Olive Tree of Vouves in Crete, Greece. Plants, 10(11), 2374. https://doi.org/10.3390/plants10112374

Friel, J., Bombarely, A., Fornell, C. D., Luque, F., & Fernández-Ocaña, A. M. (2021). Comparative Analysis of Genotyping by Sequencing and Whole-Genome Sequencing Methods in Diversity Studies of Olea europaea L. Plants, 10(11), 2514. https://doi.org/10.3390/plants10112514

Soto, M., Ramírez, L., Solana, J. C., Cook, E. C. L., Hernández-García, E., Requena, J. M., & Iborra, S. (2021). Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice. Microorganisms, 9(2), 363. https://doi.org/10.3390/microorganisms9020363

Fernández, L., Solana, J. C., Sánchez, C., Jiménez, M. Á., Requena, J. M., Coler, R., Reed, S. G., Valenzuela, J. G., Kamhawi, S., Oliveira, F., Fichera, E., Glueck, R., Bottazzi, M. E., Gupta, G., Cecilio, P., Pérez-Cabezas, B., Cordeiro-da-Silva, A., Gradoni, L., Carrillo, E., & Moreno, J. (2021). Protective Efficacy in a Hamster Model of a Multivalent Vaccine for Human Visceral Leishmaniasis (MuLeVaClin) Consisting of the KMP11, LEISH-F3+, and LJL143 Antigens in Virosomes, Plus GLA-SE Adjuvant. Microorganisms, 9(11), 2253. https://doi.org/10.3390/microorganisms9112253

Yan, H., Torchiana, F., & Bombarely, A. (2020). Guideline for genome transposon annotation derived from evaluation of popular TE identification tools. https://doi.org/10.20944/preprints202008.0275.v1

Caballero-Villalobos, L., Silva-Arias, G. A., Turchetto, C., Giudicelli, G. C., Petzold, E., Bombarely, A., & Freitas, L. B. (2020). Neutral and adaptive genomic variation in hybrid zones of two ecologically divergedPetuniaspecies (Solanaceae). Botanical Journal of the Linnean Society, 196(1), 100–122. https://doi.org/10.1093/botlinnean/boaa091

Solana, J. C., Ramírez, L., Cook, E. C., Hernández-García, E., Sacristán, S., Martín, M. E., Manuel González, V., Reguera, R. M., Balaña-Fouce, R., Fresno, M., Requena, J. M., Iborra, S., & Soto, M. (2020). Subcutaneous Immunization of Leishmania HSP70-II Null Mutant Line Reduces the Severity of the Experimental Visceral Leishmaniasis in BALB/c Mice. Vaccines, 8(1), 141. https://doi.org/10.3390/vaccines8010141

Ruiz-Márvez, E., Ramírez, C. A., Rodríguez, E. R., Flórez, M. M., Delgado, G., Guzmán, F., Gómez-Puertas, P., Requena, J. M., & Puerta, C. J. (2020). Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi. International Journal of Molecular Sciences, 21(7), 2432. https://doi.org/10.3390/ijms21072432

Sanchiz, Á., Morato, E., Rastrojo, A., Camacho, E., González-de la Fuente, S., Marina, A., Aguado, B., & Requena, J. M. (2020). The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations. Genes, 11(9), 1036. https://doi.org/10.3390/genes11091036

Hu, Y., Resende, M. F. R., Bombarely, A., Brym, M., Bassil, E., & Chambers, A. H. (2019). Genomics-based diversity analysis of Vanilla species using a Vanilla planifolia draft genome and Genotyping-By-Sequencing. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-40144-1

Manrique, S., Friel, J., Gramazio, P., Hasing, T., Ezquer, I., & Bombarely, A. (2019). Genetic insights into the modification of the pre-fertilization mechanisms during plant domestication. Journal of Experimental Botany, 70(11), 3007–3019. https://doi.org/10.1093/jxb/erz231

Soorni, A., Borna, T., Alemardan, A., Chakrabarti, M., Hunt, A. G., & Bombarely, A. (2019). Transcriptome Landscape Variation in the Genus Thymus. Genes, 10(8), 620. https://doi.org/10.3390/genes10080620

Hasing, T., Rinaldi, E., Manrique, S., Colombo, L., Haak, D. C., Zaitlin, D., & Bombarely, A. (2019). Extensive phenotypic diversity in the cultivated Florist’s Gloxinia, Sinningia speciosa (Lodd.) Hiern, is derived from the domestication of a single founder population. PLANTS, PEOPLE, PLANET, 1(4), 363–374. Portico. https://doi.org/10.1002/ppp3.10065

Talavera, A., Soorni, A., Bombarely, A., Matas, A. J., & Hormaza, J. I. (2019). Genome-Wide SNP discovery and genomic characterization in avocado (Persea americana Mill.). Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-56526-4

Rastrojo, A., Corvo, L., Lombraña, R., Solana, J. C., Aguado, B., & Requena, J. M. (2019). Analysis by RNA-seq of transcriptomic changes elicited by heat shock in Leishmania major. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-43354-9

Camacho, E., Rastrojo, A., Sanchiz, Á., González-de la Fuente, S., Aguado, B., & Requena, J. M. (2019). Leishmania Mitochondrial Genomes: Maxicircle Structure and Heterogeneity of Minicircles. Genes, 10(10), 758. https://doi.org/10.3390/genes10100758

Blankers, T., Oh, K. P., Bombarely, A., & Shaw, K. L. (2018). The Genomic Architecture of a Rapid Island Radiation: Recombination Rate Variation, Chromosome Structure, and Genome Assembly of the Hawaiian CricketLaupala. Genetics, 209(4), 1329–1344. https://doi.org/10.1534/genetics.118.300894

Ruiz, E., Ramírez, C. A., Casas, J. C., Ospina, M. I., Requena, J. M., & Puerta, C. J. (2018). Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing. Universitas Scientiarum, 23(2), 267–290. https://doi.org/10.11144/javeriana.sc23-2.cotm

Ruiz, E., Ramírez, C. A., Nocua, P., Requena, J. M., & Puerta, C. J. (2018). Identificación de proteínas reguladoras de la expresión génica en tripanosomátidos. Revista de La Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 42(165), 306. https://doi.org/10.18257/raccefyn.671

Zhao, C., Lasses, T., Bako, L., Kong, D., Zhao, B., Chanda, B., Bombarely, A., Cruz‐Ramírez, A., Scheres, B., Brunner, A. M., & Beers, E. P. (2017). XYLEM NAC DOMAIN1, an angiosperm NAC transcription factor, inhibits xylem differentiation through conserved motifs that interact with RETINOBLASTOMA‐RELATED. New Phytologist, 216(1), 76–89. Portico. https://doi.org/10.1111/nph.14704

Jiménez‐Ruiz, J., Leyva‐Pérez, M. de la O., Schilirò, E., Barroso, J. B., Bombarely, A., Mueller, L., Mercado‐Blanco, J., & Luque, F. (2017). Transcriptomic Analysis of Olea europaea L. Roots during the Verticillium dahliae Early Infection Process. The Plant Genome, 10(1). Portico. https://doi.org/10.3835/plantgenome2016.07.0060

Mizzotti, C., Galliani, B. M., Dreni, L., Sommer, H., Bombarely, A., & Masiero, S. (2017). ERAMOSA controls lateral branching in snapdragon. Scientific Reports, 7(1). https://doi.org/10.1038/srep41319

Montero‐Pau, J., Blanca, J., Bombarely, A., Ziarsolo, P., Esteras, C., Martí‐Gómez, C., Ferriol, M., Gómez, P., Jamilena, M., Mueller, L., Picó, B., & Cañizares, J. (2017). De novo assembly of the zucchini genome reveals a whole‐genome duplication associated with the origin of the Cucurbita genus. Plant Biotechnology Journal, 16(6), 1161–1171. Portico. https://doi.org/10.1111/pbi.12860

Sherman‐Broyles, S., Bombarely, A., & Doyle, J. (2017). Characterizing the allopolyploid species among the wild relatives of soybean: Utility of reduced representation genotyping methodologies. Journal of Systematics and Evolution, 55(4), 365–376. Portico. https://doi.org/10.1111/jse.12268

Montero-Pau, J., Blanca, J., Bombarely, A., Ziarsolo, P., Esteras, C., Martí-Gómez, C., Ferriol, M., Gómez, P., Jamilena, M., Mueller, L., Picó, B., & Cañizares, J. (2017). De-novoassembly of zucchini genome reveals a whole genome duplication associated with the origin of theCucurbitagenus. https://doi.org/10.1101/147702

Villarino, G. H., Hu, Q., Scanlon, M. J., Mueller, L., Bombarely, A., & Mattson, N. S. (2017). Dissecting Tissue-Specific Transcriptomic Responses from Leaf and Roots under Salt Stress in Petunia hybrida Mitchell. Genes, 8(8), 195. https://doi.org/10.3390/genes8080195

Sánchez-Sevilla, J. F., Vallarino, J. G., Osorio, S., Bombarely, A., Posé, D., Merchante, C., Botella, M. A., Amaya, I., & Valpuesta, V. (2017). Gene expression atlas of fruit ripening and transcriptome assembly from RNA-seq data in octoploid strawberry (Fragaria × ananassa). Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-14239-6

Soorni, A., Fatahi, R., Haak, D. C., Salami, S. A., & Bombarely, A. (2017). Assessment of Genetic Diversity and Population Structure in Iranian Cannabis Germplasm. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-15816-5

Soorni, A., Haak, D., Zaitlin, D., & Bombarely, A. (2017). Organelle_PBA, a pipeline for assembling chloroplast and mitochondrial genomes from PacBio DNA sequencing data. BMC Genomics, 18(1). https://doi.org/10.1186/s12864-016-3412-9

Solana, J. C., Ramírez, L., Corvo, L., de Oliveira, C. I., Barral-Netto, M., Requena, J. M., Iborra, S., & Soto, M. (2017). Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models. PLOS Neglected Tropical Diseases, 11(5), e0005644. https://doi.org/10.1371/journal.pntd.0005644

Requena, J. M., Rastrojo, A., Garde, E., López, M. C., Thomas, M. C., & Aguado, B. (2017). Genomic cartography and proposal of nomenclature for the repeated, interspersed elements of the Leishmania major SIDER2 family and identification of SIDER2-containing transcripts. Molecular and Biochemical Parasitology, 212, 9–15. https://doi.org/10.1016/j.molbiopara.2016.12.009

Requena, J. M., Rastrojo, A., Garde, E., López, M. C., Thomas, M. C., & Aguado, B. (2017). Dataset for distribution of SIDER2 elements in the Leishmania major genome and transcriptome. Data in Brief, 11, 39–43. https://doi.org/10.1016/j.dib.2017.01.001

Frazier, T. P., Palmer, N. A., Xie, F., Tobias, C. M., Donze-Reiner, T. J., Bombarely, A., Childs, K. L., Shu, S., Jenkins, J. W., Schmutz, J., Zhang, B., Sarath, G., & Zhao, B. (2016). Identification, characterization, and gene expression analysis of nucleotide binding site (NB)-type resistance gene homologues in switchgrass. BMC Genomics, 17(1). https://doi.org/10.1186/s12864-016-3201-5

Coate, J. E., Song, M. J., Bombarely, A., & Doyle, J. J. (2016). Expression‐level support for gene dosage sensitivity in three Glycine subgenus Glycine polyploids and their diploid progenitors. New Phytologist, 212(4), 1083–1093. Portico. https://doi.org/10.1111/nph.14090

Alonso, G., Rastrojo, A., López-Pérez, S., Requena, J. M., & Aguado, B. (2016). Resequencing and assembly of seven complex loci to improve the Leishmania major (Friedlin strain) reference genome. Parasites & Vectors, 9(1). https://doi.org/10.1186/s13071-016-1329-4

Fernández-Orgiler, A., Martínez-Jiménez, M. I., Alonso, A., Alcolea, P. J., Requena, J. M., Thomas, M. C., Blanco, L., & Larraga, V. (2016). A putativeLeishmaniaDNA polymerase theta protects the parasite against oxidative damage. Nucleic Acids Research, 44(10), 4855–4870. https://doi.org/10.1093/nar/gkw346

Castro, J. C., Maddox, J. D., Cobos, M., Requena, D., Zimic, M., Bombarely, A., Imán, S. A., Cerdeira, L. A., & Medina, A. E. (2015). De novo assembly and functional annotation of Myrciaria dubia fruit transcriptome reveals multiple metabolic pathways for L-ascorbic acid biosynthesis. BMC Genomics, 16(1). https://doi.org/10.1186/s12864-015-2225-6

Strickler, S. R., Bombarely, A., Munkvold, J. D., York, T., Menda, N., Martin, G. B., & Mueller, L. A. (2015). Comparative genomics and phylogenetic discordance of cultivated tomato and close wild relatives. PeerJ, 3, e793. https://doi.org/10.7717/peerj.793

Duran, I., Csukasi, F., Taylor, S. P., Krakow, D., Becerra, J., Bombarely, A., & Marí-Beffa, M. (2015). Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton. Gene Expression Patterns, 19(1–2), 60–69. https://doi.org/10.1016/j.gep.2015.07.004

Vallarino, J. G., Osorio, S., Bombarely, A., Casañal, A., Cruz‐Rus, E., Sánchez‐Sevilla, J. F., Amaya, I., Giavalisco, P., Fernie, A. R., Botella, M. A., & Valpuesta, V. (2015). Central role of Fa<scp>GAMYB</scp> in the transition of the strawberry receptacle from development to ripening. New Phytologist, 208(2), 482–496. Portico. https://doi.org/10.1111/nph.13463

Soliman, S. S. M., Greenwood, J. S., Bombarely, A., Mueller, L. A., Tsao, R., Mosser, D. D., & Raizada, M. N. (2015). An Endophyte Constructs Fungicide-Containing Extracellular Barriers for Its Host Plant. Current Biology, 25(19), 2570–2576. https://doi.org/10.1016/j.cub.2015.08.027

Requena, J. M., Montalvo, A. M., & Fraga, J. (2015). Molecular Chaperones ofLeishmania: Central Players in Many Stress-Related and -Unrelated Physiological Processes. BioMed Research International, 2015, 1–21. https://doi.org/10.1155/2015/301326

Villarino, G. H., Bombarely, A., Giovannoni, J. J., Scanlon, M. J., & Mattson, N. S. (2014). Transcriptomic Analysis of Petunia hybrida in Response to Salt Stress Using High Throughput RNA Sequencing. PLoS ONE, 9(4), e94651. https://doi.org/10.1371/journal.pone.0094651

Sherman‐Broyles, S., Bombarely, A., Powell, A. F., Doyle, J. L., Egan, A. N., Coate, J. E., & Doyle, J. J. (2014). The wild side of a major crop: Soybean’s perennial cousins from Down Under. American Journal of Botany, 101(10), 1651–1665. Portico. https://doi.org/10.3732/ajb.1400121

Bombarely, A., Coate, J. E., & Doyle, J. J. (2014). Mining transcriptomic data to study the origins and evolution of a plant allopolyploid complex. PeerJ, 2, e391. https://doi.org/10.7717/peerj.391

Kang, Y. J., Kim, S. K., Kim, M. Y., Lestari, P., Kim, K. H., Ha, B.-K., Jun, T. H., Hwang, W. J., Lee, T., Lee, J., Shim, S., Yoon, M. Y., Jang, Y. E., Han, K. S., Taeprayoon, P., Yoon, N., Somta, P., Tanya, P., Kim, K. S., … Lee, S.-H. (2014). Genome sequence of mungbean and insights into evolution within Vigna species. Nature Communications, 5(1). https://doi.org/10.1038/ncomms6443

Sherman-Broyles, S., Bombarely, A., Grimwood, J., Schmutz, J., & Doyle, J. (2014). Complete Plastome Sequences from Glycine syndetika and Six Additional Perennial Wild Relatives of Soybean. G3 Genes|Genomes|Genetics, 4(10), 2023–2033. https://doi.org/10.1534/g3.114.012690

Menda, N., Strickler, S. R., Edwards, J. D., Bombarely, A., Dunham, D. M., Martin, G. B., Mejia, L., Hutton, S. F., Havey, M. J., Maxwell, D. P., & Mueller, L. A. (2014). Analysis of wild-species introgressions in tomato inbreds uncovers ancestral origins. BMC Plant Biology, 14(1). https://doi.org/10.1186/s12870-014-0287-2

Nocua, P. A., Ramirez, C. A., Barreto, G. E., González, J., Requena, J. M., & Puerta, C. J. (2014). Leishmania braziliensis replication protein A subunit 1: molecular modelling, protein expression and analysis of its affinity for both DNA and RNA. Parasites & Vectors, 7(1). https://doi.org/10.1186/s13071-014-0573-8

Rosli, H. G., Zheng, Y., Pombo, M. A., Zhong, S., Bombarely, A., Fei, Z., Collmer, A., & Martin, G. B. (2013). Transcriptomics-based screen for genes induced by flagellin and repressed by pathogen effectors identifies a cell wall-associated kinase involved in plant immunity. Genome Biology, 14(12). https://doi.org/10.1186/gb-2013-14-12-r139

Doblas, V. G., Amorim-Silva, V., Posé, D., Rosado, A., Esteban, A., Arró, M., Azevedo, H., Bombarely, A., Borsani, O., Valpuesta, V., Ferrer, A., Tavares, R. M., & Botella, M. A. (2013). TheSUD1Gene Encodes a Putative E3 Ubiquitin Ligase and Is a Positive Regulator of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Activity inArabidopsis   . The Plant Cell, 25(2), 728–743. https://doi.org/10.1105/tpc.112.108696

D’Agostino, N., Golas, T., van de Geest, H., Bombarely, A., Dawood, T., Zethof, J., Driedonks, N., Wijnker, E., Bargsten, J., Nap, J.-P., Mariani, C., & Rieu, I. (2013). Genomic analysis of the native European Solanum species, S. dulcamara. BMC Genomics, 14(1). https://doi.org/10.1186/1471-2164-14-356

Pujar, A., Menda, N., Bombarely, A., Edwards, J. D., Strickler, S. R., & Mueller, L. A. (2013). From manual curation to visualization of gene families and networks across Solanaceae plant species. Database, 2013. https://doi.org/10.1093/database/bat028

Jung, S., Ficklin, S. P., Lee, T., Cheng, C.-H., Blenda, A., Zheng, P., Yu, J., Bombarely, A., Cho, I., Ru, S., Evans, K., Peace, C., Abbott, A. G., Mueller, L. A., Olmstead, M. A., & Main, D. (2013). The Genome Database for Rosaceae (GDR): year 10 update. Nucleic Acids Research, 42(D1), D1237–D1244. https://doi.org/10.1093/nar/gkt1012

Ramírez, C. A., Dea-Ayuela, M. A., Gutiérrez-Blázquez, M. D., Bolas-Fernández, F., Requena, J. M., & Puerta, C. J. (2013). Identification of proteins interacting with HSP70 mRNAs in Leishmania braziliensis. Journal of Proteomics, 94, 124–137. https://doi.org/10.1016/j.jprot.2013.09.008

Prada, C. F., Álvarez-Velilla, R., Balaña-Fouce, R., Prieto, C., Calvo-Álvarez, E., Escudero-Martínez, J. M., Requena, J. M., Ordóñez, C., Desideri, A., Pérez-Pertejo, Y., & Reguera, R. M. (2013). Gimatecan and other camptothecin derivatives poison Leishmania DNA-topoisomerase IB leading to a strong leishmanicidal effect. Biochemical Pharmacology, 85(10), 1433–1440. https://doi.org/10.1016/j.bcp.2013.02.024

Rastrojo, A., Carrasco-Ramiro, F., Martín, D., Crespillo, A., Reguera, R. M., Aguado, B., & Requena, J. M. (2013). The transcriptome of Leishmania majorin the axenic promastigote stage: transcript annotation and relative expression levels by RNA-seq. BMC Genomics, 14(1). https://doi.org/10.1186/1471-2164-14-223

Ramírez, C. A., Requena, J. M., & Puerta, C. J. (2013). Alpha tubulin genes from Leishmania braziliensis:genomic organization, gene structure and insights on their expression. BMC Genomics, 14(1). https://doi.org/10.1186/1471-2164-14-454

Fraga, J., Montalvo, A. M., Van der Auwera, G., Maes, I., Dujardin, J.-C., & Requena, J. M. (2013). Evolution and species discrimination according to the Leishmania heat-shock protein 20 gene. Infection, Genetics and Evolution, 18, 229–237. https://doi.org/10.1016/j.meegid.2013.05.020

(2012). The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 485(7400), 635–641. https://doi.org/10.1038/nature11119

Lima-Silva, V., Rosado, A., Amorim-Silva, V., Muñoz-Mérida, A., Pons, C., Bombarely, A., Trelles, O., Fernández-Muñoz, R., Granell, A., Valpuesta, V., & Botella, M. Á. (2012). Genetic and genome-wide transcriptomic analyses identify co-regulation of oxidative response and hormone transcript abundance with vitamin C content in tomato fruit. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-187

Bombarely, A., Edwards, K. D., Sanchez-Tamburrino, J., & Mueller, L. A. (2012). Deciphering the complex leaf transcriptome of the allotetraploid species Nicotiana tabacum: a phylogenomic perspective. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-406

Strickler, S. R., Bombarely, A., & Mueller, L. A. (2012). Designing a transcriptome next‐generation sequencing project for a nonmodel plant species1. American Journal of Botany, 99(2), 257–266. Portico. https://doi.org/10.3732/ajb.1100292

Bombarely, A., Rosli, H. G., Vrebalov, J., Moffett, P., Mueller, L. A., & Martin, G. B. (2012). A Draft Genome Sequence ofNicotiana benthamianato Enhance Molecular Plant-Microbe Biology Research. Molecular Plant-Microbe Interactions®, 25(12), 1523–1530. https://doi.org/10.1094/mpmi-06-12-0148-ta

Guo, S., Zhang, J., Sun, H., Salse, J., Lucas, W. J., Zhang, H., Zheng, Y., Mao, L., Ren, Y., Wang, Z., Min, J., Guo, X., Murat, F., Ham, B.-K., Zhang, Z., Gao, S., Huang, M., Xu, Y., Zhong, S., … Xu, Y. (2012). The draft genome of watermelon (Citrullus lanatus) and resequencing of 20 diverse accessions. Nature Genetics, 45(1), 51–58. https://doi.org/10.1038/ng.2470

Balaña-Fouce, R., Prada, C. F., Requena, J. M., Cushman, M., Pommier, Y., Álvarez-Velilla, R., Escudero-Martínez, J. M., Calvo-Álvarez, E., Pérez-Pertejo, Y., & Reguera, R. M. (2012). Indotecan (LMP400) and AM13-55: Two Novel Indenoisoquinolines Show Potential for Treating Visceral Leishmaniasis. Antimicrobial Agents and Chemotherapy, 56(10), 5264–5270. https://doi.org/10.1128/aac.00499-12

Calvo-Álvarez, E., Guerrero, N. A., Álvarez-Velilla, R., Prada, C. F., Requena, J. M., Punzón, C., Llamas, M. Á., Arévalo, F. J., Rivas, L., Fresno, M., Pérez-Pertejo, Y., Balaña-Fouce, R., & Reguera, R. M. (2012). Appraisal of a Leishmania major Strain Stably Expressing mCherry Fluorescent Protein for Both In Vitro and In Vivo Studies of Potential Drugs and Vaccine against Cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, 6(11), e1927. https://doi.org/10.1371/journal.pntd.0001927

Requena, J. M., Chicharro, C., García, L., Parrado, R., Puerta, C. J., & Cañavate, C. (2012). Sequence analysis of the 3’-untranslated region of HSP70 (type I) genes in the genus Leishmania: its usefulness as a molecular marker for species identification. Parasites & Vectors, 5(1). https://doi.org/10.1186/1756-3305-5-87

Ali, N., Mekuria, A. H., Requena, J. M., & Engwerda, C. (2012). Immunity to Visceral Leishmaniasis. Journal of Tropical Medicine, 2012, 1–2. https://doi.org/10.1155/2012/780809

Privat, I., Bardil, A., Gomez, A. B., Severac, D., Dantec, C., Fuentes, I., Mueller, L., Joët, T., Pot, D., Foucrier, S., Dussert, S., Leroy, T., Journot, L., de Kochko, A., Campa, C., Combes, M.-C., Lashermes, P., & Bertrand, B. (2011). The “PUCE CAFE” Project: the First 15K Coffee Microarray, a New Tool for Discovering Candidate Genes correlated to Agronomic and Quality Traits. BMC Genomics, 12(1). https://doi.org/10.1186/1471-2164-12-5

Osorio, S., Bombarely, A., Giavalisco, P., Usadel, B., Stephens, C., Aragüez, I., Medina-Escobar, N., Botella, M. A., Fernie, A. R., & Valpuesta, V. (2011). Demethylation of oligogalacturonides by FaPE1 in the fruits of the wild strawberry Fragaria vesca triggers metabolic and transcriptional changes associated with defence and development of the fruit. Journal of Experimental Botany, 62(8), 2855–2873. https://doi.org/10.1093/jxb/erq465

Requena, J., M. (2011). Lights and shadows on gene organization and regulation of gene expression in Leishmania. Frontiers in Bioscience, 16(1), 2069. https://doi.org/10.2741/3840

Carrión, J., Folgueira, C., Soto, M., Fresno, M., & Requena, J. M. (2011). Leishmania infantum HSP70-II null mutant as candidate vaccine against leishmaniasis: a preliminary evaluation. Parasites & Vectors, 4(1). https://doi.org/10.1186/1756-3305-4-150

Ramírez, C. A., Requena, J. M., & Puerta, C. J. (2011). Identification of the HSP70-II gene in Leishmania braziliensis HSP70 locus: genomic organization and UTRs characterization. Parasites & Vectors, 4(1). https://doi.org/10.1186/1756-3305-4-166

Edwards, K. D., Bombarely, A., Story, G. W., Allen, F., Mueller, L. A., Coates, S. A., & Jones, L. (2010). TobEA: an atlas of tobacco gene expression from seed to senescence. BMC Genomics, 11(1), 142. https://doi.org/10.1186/1471-2164-11-142

Bombarely, A., Merchante, C., Csukasi, F., Cruz-Rus, E., Caballero, J. L., Medina-Escobar, N., Blanco-Portales, R., Botella, M. A., Muñoz-Blanco, J., Sánchez-Sevilla, J. F., & Valpuesta, V. (2010). Generation and analysis of ESTs from strawberry (Fragaria xananassa) fruits and evaluation of their utility in genetic and molecular studies. BMC Genomics, 11(1). https://doi.org/10.1186/1471-2164-11-503

Bombarely, A., Menda, N., Tecle, I. Y., Buels, R. M., Strickler, S., Fischer-York, T., Pujar, A., Leto, J., Gosselin, J., & Mueller, L. A. (2010). The Sol Genomics Network (solgenomics.net): growing tomatoes using Perl. Nucleic Acids Research, 39(Database), D1149–D1155. https://doi.org/10.1093/nar/gkq866

Folgueira, C., Martinez-Bonet, M., & Requena, J. M. (2010). The Leishmania infantum PUF proteins are targets of the humoral response during visceral leishmaniasis. BMC Research Notes, 3(1), 13. https://doi.org/10.1186/1756-0500-3-13

Ramírez, C., Puerta, C., & Requena, J. M. (2010). Evidence of RNA editing in Leishmania braziliensis promastigotes. Parasitology Research, 108(3), 731–739. https://doi.org/10.1007/s00436-010-2190-6

Mueller, L. A., Lankhorst, R. K., Tanksley, S. D., Giovannoni, J. J., White, R., Vrebalov, J., Fei, Z., van Eck, J., Buels, R., Mills, A. A., Menda, N., Tecle, I. Y., Bombarely, A., Stack, S., Royer, S. M., Chang, S., Shearer, L. A., Kim, B. D., Jo, S., … Stiekema, W. (2009). A Snapshot of the Emerging Tomato Genome Sequence. The Plant Genome, 2(1). Portico. https://doi.org/10.3835/plantgenome2008.08.0005

Carrillo, E., Crusat, M., Nieto, J., Chicharro, C., Thomas, M. del C., Martínez, E., Valladares, B., Cañavate, C., Requena, J. M., López, M. C., Alvar, J., & Moreno, J. (2008). Immunogenicity of HSP-70, KMP-11 and PFR-2 leishmanial antigens in the experimental model of canine visceral leishmaniasis. Vaccine, 26(15), 1902–1911. https://doi.org/10.1016/j.vaccine.2008.01.042

Folgueira, C., & Carrión, J. (2008). Effects of the disruption of the HSP70-II gene on the growth, morphology, and virulence of Leishmania infantum promastigotes [JB]. International Microbiology, 11, 81–89. https://doi.org/10.2436/20.1501.01.47

Requena, J. M., Folgueira, C., López, M. C., & Thomas, M. C. (2008). The SIDER2 elements, interspersed repeated sequences that populate the Leishmania genomes, constitute subfamilies showing chromosomal proximity relationship. BMC Genomics, 9(1), 263. https://doi.org/10.1186/1471-2164-9-263

Folgueira, C., & Requena, J. M. (2007). A postgenomic view of the heat shock proteins in kinetoplastids. FEMS Microbiology Reviews, 31(4), 359–377. https://doi.org/10.1111/j.1574-6976.2007.00069.x

Folgueira, C., & Requena, J. M. (2007). Pitfalls of the CAT reporter gene for analyzing translational regulation in Leishmania. Parasitology Research, 101(5), 1449–1452. https://doi.org/10.1007/s00436-007-0640-6

CARRIÓN, J., NIETO, A., IBORRA, S., INIESTA, V., SOTO, M., FOLGUEIRA, C., ABANADES, D. R., REQUENA, J. M., & ALONSO, C. (2006). Immunohistological features of visceral leishmaniasis in BALB/c mice. Parasite Immunology, 28(5), 173–183. Portico. https://doi.org/10.1111/j.1365-3024.2006.00817.x

FOLGUEIRA, C., CAÑAVATE, C., CHICHARRO, C., & REQUENA, J. M. (2006). Genomic organization and expression of the HSP70 locus in New and Old World Leishmania species. Parasitology, 134(3), 369–377. https://doi.org/10.1017/s0031182006001570

Quijada, L., Soto, M., & Requena, J. M. (2005). Genomic DNA macroarrays as a tool for analysis of gene expression in Leishmania. Experimental Parasitology, 111(1), 64–70. https://doi.org/10.1016/j.exppara.2005.04.006

Folgueira, C., Quijada, L., Soto, M., Abanades, D. R., Alonso, C., & Requena, J. M. (2005). The Translational Efficiencies of the Two Leishmania infantum HSP70 mRNAs, Differing in Their 3′-Untranslated Regions, Are Affected by Shifts in the Temperature of Growth through Different Mechanisms. Journal of Biological Chemistry, 280(42), 35172–35183. https://doi.org/10.1074/jbc.m505559200

Parody, N., Soto, M., Requena, J. M., & Alonso, C. (2004). Adjuvant guided polarization of the immune humoral response against a protective multicomponent antigenic protein (Q) from Leishmania infantum. A CpG + Q mix protects Balb/c mice from infection. Parasite Immunology, 26(6–7), 283–293. Portico. https://doi.org/10.1111/j.0141-9838.2004.00711.x

SOTO, M., IBORRA, S., QUIJADA, L., FOLGUEIRA, C., ALONSO, C., & REQUENA, J. M. (2004). Cell-cycle-dependent translation of histone mRNAs is the key control point for regulation of histone biosynthesis in Leishmania infantum. Biochemical Journal, 379(3), 617–625. https://doi.org/10.1042/bj20031522

Larreta, R., Soto, M., Quijada, L., Folgueira, C., Abanades, D. R., Alonso, C., & Requena, J. M. (2004). The expression of HSP83 genes in Leishmania infantum is affected by temperature and by stage-differentiation and is regulated at the levels of mRNA stability and translation. BMC Molecular Biology, 5(1). https://doi.org/10.1186/1471-2199-5-3

IBORRA, S. (2004). Vaccination with a plasmid DNA cocktail encoding the nucleosomal histones of Leishmania confers protection against murine cutaneous leishmaniosis. Vaccine, 22(29–30), 3865–3876. https://doi.org/10.1016/j.vaccine.2004.04.015

Requena, J. M., Iborra, S., Carrión, J., Alonso, C., & Soto, M. (2004). Recent advances in vaccines for leishmaniasis. Expert Opinion on Biological Therapy, 4(9), 1505–1517. https://doi.org/10.1517/14712598.4.9.1505

Villa, H., Pérez‐Pertejo, Y., García‐Estrada, C., Reguera, R. M., Requena, J. M., Tekwani, B. L., Balaña‐Fouce, R., & Ordóñez, D. (2003). Molecular and functional characterization of adenylate kinase 2 gene fromLeishmania donovani. European Journal of Biochemistry, 270(21), 4339–4347. Portico. https://doi.org/10.1046/j.1432-1033.2003.03826.x

SOTO, M., QUIJADA, L., LARRETA, R., IBORRA, S., ALONSO, C., & REQUENA, J. M. (2003). Leishmania infantum possesses a complex family of histone H2A genes: structural characterization and analysis of expression. Parasitology, 127(2), 95–105. https://doi.org/10.1017/s0031182003003445

García-Estrada, C. (2003). Identification of a gene in Leishmania infantum encoding a protein that contains a SP-RING/MIZ zinc finger domain. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1629(1–3), 44–52. https://doi.org/10.1016/j.bbaexp.2003.07.001

Molano, I., Alonso, M. G., Mirón, C., Redondo, E., Requena, J. M., Soto, M., Nieto, C. G., & Alonso, C. (2003). A Leishmania infantum multi-component antigenic protein mixed with live BCG confers protection to dogs experimentally infected with L. infantum. Veterinary Immunology and Immunopathology, 92(1–2), 1–13. https://doi.org/10.1016/s0165-2427(02)00315-x

Requena, J. M., Quijada, L., Soto, M., & Alonso, C. (2003). Conserved nucleotides surrounding the trans-splicing acceptor site and the translation initiation codon in Leishmania genes. Experimental Parasitology, 103(1–2), 78–81. https://doi.org/10.1016/s0014-4894(03)00061-4

Quijada, L., Soto, M., Alonso, C., & Requena, J. M. (2003). High-efficiency plating method for Leishmania infantum. Molecular and Biochemical Parasitology, 130(2), 139–141. https://doi.org/10.1016/s0166-6851(03)00159-2

Larreta, R. (2002). Antigenic properties of the Leishmania infantum GRP94 and mapping of linear B-cell epitopes. Immunology Letters, 80(3), 199–205. https://doi.org/10.1016/s0165-2478(01)00331-5

Echeverria, P., Dran, G., Pereda, G., Rico, A. I., Requena, J. M., Alonso, C., Guarnera, E., & Angel, S. O. (2001). Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination. Immunology Letters, 76(2), 107–110. https://doi.org/10.1016/s0165-2478(01)00179-1

Soto, M., Alonso, C., & Requena, J. M. (2000). TheLeishmania infantumacidic ribosomal protein LiP2a induces a prominent humoral responsein vivoand stimulates cell proliferationin vitroand interferon-gamma (IFN-γ) production by murine splenocytes. Clinical and Experimental Immunology, 122(2), 212–218. https://doi.org/10.1046/j.1365-2249.2000.01372.x

Larreta, R., Soto, M., Alonso, C., & Requena, J. M. (2000). Leishmania infantum: Gene Cloning of the GRP94 Homologue, Its Expression as Recombinant Protein, and Analysis of Antigenicity. Experimental Parasitology, 96(2), 108–115. https://doi.org/10.1006/expr.2000.4553

Requena, J. M., Soto, M., Doria, M. D., & Alonso, C. (2000). Immune and clinical parameters associated with Leishmania infantum infection in the golden hamster model. Veterinary Immunology and Immunopathology, 76(3–4), 269–281. https://doi.org/10.1016/s0165-2427(00)00221-x

Quijada, L., Soto, M., Alonso, C., & Requena, J. M. (2000). Identification of a putative regulatory element in the 3′-untranslated region that controls expression of HSP70 in Leishmania infantum. Molecular and Biochemical Parasitology, 110(1), 79–91. https://doi.org/10.1016/s0166-6851(00)00258-9

SOTO, M., QUIJADA, L., ALONSO, C., & REQUENA, J. M. (2000). Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control. Biochemical Journal, 346(1), 99–105. https://doi.org/10.1042/bj3460099

Requena, J. M., Alonso, C., & Soto, M. (2000). Evolutionarily Conserved Proteins as Prominent Immunogens during Leishmania Infections. Parasitology Today, 16(6), 246–250. https://doi.org/10.1016/s0169-4758(00)01651-3

Planelles, L., Marañón, C., Requena, J. M., & López, M. C. (1999). Phage Recovery by Electroporation of Naked DNA into Host Cells Avoids the Use of Packaging Extracts. Analytical Biochemistry, 267(1), 234–235. https://doi.org/10.1006/abio.1998.2963

Rico, A. I., Angel, S. O., Alonso, C., & Requena, J. M. (1999). Immunostimulatory properties of the Leishmania infantum heat shock proteins HSP70 and HSP83. Molecular Immunology, 36(17), 1131–1139. https://doi.org/10.1016/s0161-5890(99)00136-4

SOTO, M., REQUENA, J. M., QUIJADA, L., PEREZ, M. J., NIETO, C. G., GUZMAN, F., PATARROYO, M. E., & ALONSO, C. (1999). Antigenicity of theLeishmania infantumhistones H2B and H4 during canine viscerocutaneous leishmaniasis. Clinical and Experimental Immunology, 115(2), 342–349. https://doi.org/10.1046/j.1365-2249.1999.00796.x

Nieto, C. G., Garcı́a-Alonso, M., Requena, J. M., Mirón, C., Soto, M., Alonso, C., & Navarrete, I. (1999). Analysis of the humoral immune response against total and recombinant antigens of Leishmania infantum: correlation with disease progression in canine experimental leishmaniasis. Veterinary Immunology and Immunopathology, 67(2), 117–130. https://doi.org/10.1016/s0165-2427(98)00213-x

Carriazo, C. S., Sembaj, A., Aguerri, A. M., Requena, J. M., Alonso, C., Búa, J., Ruiz, A., Segura, E., & Barral, J. M. (1998). Polymerase Chain Reaction Procedure to Detect Trypanosoma cruzi in Blood Samples from Chronic Chagasic Patients. Diagnostic Microbiology and Infectious Disease, 30(3), 183–186. https://doi.org/10.1016/s0732-8893(97)00238-1

Jiménez‐Ruiz, A., Boceta, C., Bonay, P., Requena, J. M., & Alonso, C. (1998). Cloning, sequencing, and expression of the PSA genes from Leishmania infantum. European Journal of Biochemistry, 251(1–2), 389–397. Portico. https://doi.org/10.1046/j.1432-1327.1998.2510389.x

Quijada, L. (1998). Analysis of the antigenic properties of the L. infantum Hsp70: design of synthetic peptides for specific serodiagnosis of human leishmaniasis. Immunology Letters, 63(3), 169–174. https://doi.org/10.1016/s0165-2478(98)00071-6

Soto, M., Quijada, L., Alonso, C., & Requena, J. M. (1997). Molecular cloning and analysis of expression of the Leishmania infantum histone H4 genes1Note: Nucleotide sequence data reported in this paper have been submitted to the EMBL/GenBank/DDBJ databases with the accession numbers Y13915 (cDNA LiH4-1) and Y13916 (cDNA LiH4-2).1. Molecular and Biochemical Parasitology, 90(2), 439–447. https://doi.org/10.1016/s0166-6851(97)00178-3

Berberich, C., Requena, J. M., & Alonso, C. (1997). Cloning of Genes and Expression and Antigenicity Analysis of theLeishmania infantumKMP-11 Protein. Experimental Parasitology, 85(1), 105–108. https://doi.org/10.1006/expr.1996.4120

ARAYA, J., CANO, M. I., GOMES, H. B. M., NOVAK, E. M., REQUENA, J. M., ALONSO, C., LEVIN, M. J., GUEVARA, P., RAMIREZ, J. L., & DA SILVEIRA, J. F. (1997). Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi. Parasitology, 115(6), 563–570. https://doi.org/10.1017/s0031182097001674

Quijada, L., Soto, M., Alonso, C., & Requena, J. M. (1997). Analysis of Post-transcriptional Regulation Operating on Transcription Products of the Tandemly Linked Leishmania infantum hsp70 Genes. Journal of Biological Chemistry, 272(7), 4493–4499. https://doi.org/10.1074/jbc.272.7.4493

Requena, J. M., Soto, M., Quijada, L., Carrillo, G., & Alonso, C. (1997). A region containing repeated elements is associated with transcriptional termination of Leishmania infantum ribosomal RNA genes1Note: Nucleotide sequences data reported in this paper are available in the EMBL, GenBank™ and DDJB data bases under the accession number X99114.1. Molecular and Biochemical Parasitology, 84(1), 101–110. https://doi.org/10.1016/s0166-6851(96)02785-5

Requena, J. M., López, M. C., & Alonso, C. (1996). Genomic repetitive DNA elements of Trypanosoma cruzi. Parasitology Today, 12(7), 279–283. https://doi.org/10.1016/0169-4758(96)10024-7

Angel, S. O., Requena, J. M., Soto, M., Criago, D., & Alonso, C. (1996). During canine leishmaniasis a protein belonging to the 83-kDa heat-shock protein family elicits a strong humoral response. Acta Tropica, 62(1), 45–56. https://doi.org/10.1016/s0001-706x(96)00020-4

Cano, M. I., Gruber, A., Vazquez, M., Cortés, A., Levin, M. J., González, A., Degrave, W., Rondinelli, E., Zingales, B., Ramirez, J., Alonso, C., Requena, JoséM., & Silveira, J. da. (1995). Molecular karyotype of clone CL Brener chosen for the Trypanosoma cruzi Genome Project. Molecular and Biochemical Parasitology, 71(2), 273–278. https://doi.org/10.1016/0166-6851(95)00066-a

Soto, M., Requena, J. M., Quijada, L., García, M., Guzman, F., Patarroyo, M. E., & Alonso, C. (1995). Mapping of the linear antigenic determinants from the Leishmania infantum histone H2A recognized by sera from dogs with leishmaniasis. Immunology Letters, 48(3), 209–214. https://doi.org/10.1016/0165-2478(95)02473-5

Soto, M., Requena, J. M., Quijada, L., Guzman, F., Patarroyo, M. E., & Alonso, C. (1995). Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis. Immunology Letters, 48(1), 23–28. https://doi.org/10.1016/0165-2478(95)02436-0

Soto, M., Requena, J. M., Moreira, D., & Alonso, C. (1995). Assignment of genes toLeishmania infantumchromosomes: karyotype and ploidy. FEMS Microbiology Letters, 129(1), 27–32. https://doi.org/10.1111/j.1574-6968.1995.tb07552.x

Soto, M., Requena, JoséM., Morales, G., & Alonso, C. (1994). The Leishmania infantum histone H3 possesses an extremely divergent N-terminal domain. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1219(2), 533–535. https://doi.org/10.1016/0167-4781(94)90082-5

Requena, J. M., Martin, F., Soto, M., López, M. C., & Alonso, C. (1994). Characterization of a short interspersed reiterated DNA sequence of Trypanosoma cruzi located at the 3′-end of a poly(A)+ transcript. Gene, 146(2), 245–250. https://doi.org/10.1016/0378-1119(94)90300-x

Requena, J. M., Soto, M., Guzman, F., Maekelt, A., Noya, O., Patarroyo, M. E., & Alonso, C. (1993). Mapping of antigenic determinants of the T. cruzi HSP70 in chagasic and healthy individuals. Molecular Immunology, 30(12), 1115–1121. https://doi.org/10.1016/0161-5890(93)90158-8

Soto, M., Requena, J. M., & Alonso, C. (1993). Isolation, characterization and analysis of the expression of the Leishmania ribosomal PO protein genes. Molecular and Biochemical Parasitology, 61(2), 265–274. https://doi.org/10.1016/0166-6851(93)90072-6

Martin, F., Requena, J. M., Martin, J., Alonso, C., & Lopez, M. C. (1993). Cytoplasmic-Nuclear Translocation of the HSP70 Protein during Environmental Stress in Trypanosoma cruzi. Biochemical and Biophysical Research Communications, 196(3), 1155–1162. https://doi.org/10.1006/bbrc.1993.2372

Pérez, J. M., Requena, J. M., Craciunescu, D., Doadrio, J. C., & Alonso, C. (1993). Binding of Pt-pentamidine to nucleosomal DNA. Studies of the antiproliferative activity of the drug against human cancer cells. Chemico-Biological Interactions, 89(1), 61–72. https://doi.org/10.1016/0009-2797(93)03199-5

Requena, J. M., Soto, M., & Alonso, C. (1993). Bacteriophage λ: long-term stored stocks ready for lysis. Trends in Genetics, 9(1), 4. https://doi.org/10.1016/0168-9525(93)90052-j

Requena, JoséM., Jimenez-Ruiz, A., Soto, M., Assiego, R., Santarén, J. F., Lopez, M. C., Patarroyo, M. E., & Alonso, C. (1992). Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase. Molecular and Biochemical Parasitology, 53(1–2), 201–211. https://doi.org/10.1016/0166-6851(92)90022-c

SOTO, M., REQUENA, J. M., GOMEZ, L. C., NAVARRETE, I., & ALONSO, C. (1992). Molecular characterization of a Leishmania donovani infantum antigen identified as histone H2A. European Journal of Biochemistry, 205(1), 211–216. Portico. https://doi.org/10.1111/j.1432-1033.1992.tb16770.x

Requena, J. M., Jimenez-Ruiz, A., Soto, M., Lopez, M. C., & Alonso, C. (1992). Characterization of a highly repeated interspersed DNA sequence of Trypanosoma cruzi: its potential use in diagnosis and strain classification. Molecular and Biochemical Parasitology, 51(2), 271–280. https://doi.org/10.1016/0166-6851(92)90077-w

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